PMC

Competitive inhibition with various concentrations of polysaccharide. The competitive inhibition assay is shown to be sensitive, with an increase in inhibition being seen with increasing polysaccharide concentrations.

A. Double-reciprocal plot for E-3a. All data sets converge upon a common y- intercept, supporting a competitive mechanism of inhibition. B. Double-reciprocal plot for E-3c also follows a competitive model. C. Double-reciprocal plot for E-3t, depicting competitive inhibition. D. Double-reciprocal plot for E-3w supports a competitive model of inhibition.

Lineweaver–Burk plots: competitive and non-competitive nature of GST inhibition. The experiments were performed on three different days using freshly prepared GST, CDNB, CAPE, GSH and tyrosinase stock solutions on a daily basis. The average of data points was used to graph the Lineweaver–Burk plot to determine the nature of competitive and non-competitive inhibition of GST. (A) Mixed GST inhibition by CAPE with respect to CDNB. (B) Mixed GST inhibition by CAPE-quinone with respect to CDNB. (C) Competitive GST inhibition by CAPE-SG conjugate with respect to CDNB. (D) Competitive GST inhibition by ethacrynic acid (EA) with respect to CDNB. (E) Mixed GST inhibition by CAPE with respect to GSH. (F) Competitive GST inhibition by CAPE-quinone with respect to GSH. (G) Mixed GST inhibition by CAPE-SG conjugate with respect to GSH. (H) Non-competitive GST inhibition by ethacrynic acid (EA) with respect to GSH. The unit of GST activity is µM/min (nmol/mL/min).

Kinetic plots for arginase 1 and arginase 2 inhibition by HOMOARG. Error bars represent the standard error of the mean. (A) Arginase 1 inhibition by HOMOARG IC₅₀ plot. Each data point is the mean of two singlicate experiments, (B) Arginase 1 inhibition by HOMOARG K_i plot. Each data point is the mean of three singlicate experiments and the competitive model fit is represented as solid lines, (C) Representative Dixon plot of the inhibition of arginase 1 by HOMOARG. Each data point is the mean of three singlicate experiments and the competitive model fit is shown as solid lines, (D) Arginase 2 inhibition by HOMOARG IC₅₀ plot. Each data point is the mean of two singlicate experiments, (E) Arginase 2 inhibition by HOMOARG K_i plot. Each data point is the mean of three singlicate experiments and the competitive model fit is shown as solid lines, (F) Representative Dixon plot of the inhibition of arginase 2 by HOMOARG. Each data point is the mean of three singlicate experiments and the competitive model fit is represented as solid lines.